The solvent shipping system consists of a pump, through which solvent (cell section) is shipped at a managed movement fee. If air will get dissolved from the cellular phase, it may well make air bubbles that fluctuate the flow fee.
Integrator is the computer-primarily based facts processor utilized to history the electronic sign. Straightforward to specially made application is formulated for HPLC.
試料を注入する部分で、手動式（マニュアルインジェクター）と自動式（オートインジェクター）がある。
Changing the cell section’s polarity index modifications a solute’s retention factor. As we uncovered in Chapter 12.three, on the other hand, a transform in k is not really a successful way to boost resolution when the Original worth of k is bigger than 10.
イオン交換クロマトグラフィーでは、無機イオンや高極性分子を電荷を利用して分離する。陽イオンタイプと陰イオンタイプの両方がある。イオン交換樹脂を利用する。
Fluoxetine is an additional name for that antidepressant drug Prozac. The dedication of fluoxetine in serum is a crucial Component of monitoring its therapeutic use.
A pulse damper is a chamber crammed with an quickly compressed fluid and a versatile diaphragm. Through the piston’s forward stroke the fluid in the heartbeat damper is compressed. If the piston withdraws to refill the pump, strain from the expanding fluid in the heartbeat damper maintains the circulation price.
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Resolute® BioSMB System is often a multi-column chromatography system meant to be deployed as Element of a standard batch course of action or high performance liquid chromatography simply a continuous downstream procedure and is an ideal Remedy for those in search of decreased generation prices and limit resin utilization with small disruption to present-day chromatography approaches.
원하는 분석 결과를 얻기 위해서는 컬럼도 충분히 고려하고 선택하는 것이 좋습니다.
Sample injection introduces the prepared sample in the HPLC system. The injection volume and system can appreciably influence:
Compounds in the sample partition involving the stationary stage along more info with the mobile period in partition chromatography. Compounds having a more robust affinity to the stationary stage invest more time interacting with it, leading to slower elution with the column.
검토 중에서 컬럼이나 이동상 등 여러 조건의 조합은 분석 가능성의 큰 영향을 미칩니다.)
The injector is positioned once the pump to introduce the sample in the cellular phase. Syringes are essentially the most typical sample injectors. From the automobile-injector, injection of your sample occurs mechanically at the predetermined time.